Assistant Professor

Ph.D. University of Georgia, Athens, GA
B.S. Georgia College, Milledgeville, GA

Emailklabbott@uams.edu
Office:  (501)686-7575 – Winthrop P. Rockefeller Cancer Institute, Rm. 11-111
Labs:  (501)686-7579 – Winthrop P. Rockefeller Cancer Institute, Rms. 11-156 & 11-158
Fax:  (501)-526-5812

Biomedical glycomics, proteomics, and cell biology studies of human cancers.

Our lab is interested in the study of glycans (sugars) that are involved in cellular differentiation and cancer progression in a field of study known as glycomics. The posttranslational modification of proteins with glycans changes significantly during development and in the presence of diseases such as cancer. These glycan modifications play important roles in establishing the functions of proteins. We are applying glycoproteomic techniques to identify glycoproteins with tumor-specific glycosaylation changes in a variety of human cancers. These glycoprotein biomarkers can be evaluated for use as diagnostic and/or therapeutic targets.

Selected Publications:

1. Zhao P, Nairn A, Hester S, Moremen K, O’Regan R, Oprea G, Wells L, Pierce M, and Abbott KL (2012) Proteomic identification of glycosylphosphatidylinositol anchor-dependent membrane proteins elevated in breast carcinoma. J. Biol. Chem. 287(30):25230-40.[PMID: 22654114].

2. Dengaj D, Abbott KL, Mookerjee A, Zhao A, Kirby PS, Sandaltzopoulos R, Powell DJ, Siegel DL, Wolf C, and Scholler N (2011) Mannose receptor engagement by mesothelin GPI anchor polarizestumor associated macrophages and is blocked by a recombinant antibody directed against mannose eceptor domain 4. PLoS One. 6 (12): e28386. [PMID:22163010]

3. Abbott KL, Lim J, Wells L, Benigno BB, McDonald JF, Pierce M (2010) Identification of candidate biomarkers with cancer-specific glycosylation in the tissue and serum of endometrioid ovarian cancer patients by glycoproteomic analysis. Proteomics 10: 470-481. [PMID: 19953551]

4. Abbott KL (2010) Glycomic analysis of ovarian cancer: past, present, and future. Cancer Biomarkers. 8 (4-5):273-80. [PMID: 22045358].

5. Abbott KL, Pierce JM (2010) Lectin-based glycoproteomic techniques for the enrichment and identification of potential biomarkers. Methods Enzymol 480:461-76. [PMID:20816222]

6. Abbott KL, Nairn AV, Hall EM, Horton, MB, Walker D, McDonald JF, Moremen KW, Dinulescu DM, Pierce M (2008) Focused glycomic analysis for the N-linked glycan pathway in ovarian cancer. Proteomics 8: 3210-3220 (Selected for journal cover). [PMID: 18690643]

7. Abbott KL, Aoki K, Lim J, Perlman M, Johnson R, O’Regan RM, Wells L, Tiemeyer M, Pierce M (2008) Targeted glycoproteomic identification of biomarkers for human breast carcinoma. J. Proteome Res. 7: 1470-1480. [PMID: 18271524]

8. Abbott KL, Troupe K, Lee I, Pierce M. “Integrin-dependent neuroblastoma cell adhesion and migration on laminin is regulated by expression levels of two enzymes in the O-mannosyl-linked glycosylation pathway, PomGnT1 and GnT-Vb” Exp Cell Res. 312(15):2837-50 (2006).

9. Lee I, Guo HB, Kamar M, Abbott K, Troupe K, Lee JK, Alvarez-Manilla G, Pierce M. “N-acetylglucosaminyltransferase VB expression enhances beta 1 integrin-dependent PC12 neurite outgrowth on laminin and collagen” J. Neurochem, 97(4) 947-56 (2006).

10. Abbott KL (2010) Glycomic analysis of ovarian cancer: past, present, and future. Cancer Biomarkers. 8 (4-5):273-80. [PMID: 22045358].

11. Allam H, Aoki K, McDonald JF, MacIntosh SG, Tiemeyer M, Abbott KL *(2015) “Glycomic analysis of membrane glycoproteins with bisecting glycosylation from ovarian cancer tissues reveal novel structures and functions.” J Proteome Res. 14(1):434-46.[PMID 25437919] [PMC 4286206]

12. Guo H, Abbott KL.*( 2015)“Functional impact of tumor-specific N-linked glycan changes in breast and ovarian cancers”. Adv. Cancer Res. 126:281-303[PMID 25727151]

13. Dolezal S, Hester S, Kirby PS, Nairn A, Pierce M, Abbott KL*. (2014) “Elevated levels of glycosylphosphatidylinositol (GPI) anchored proteins in plasma from human cancers detected by C.septicum alpha toxin”. Cancer Biomark. 14(1):55-62. [PMID 24643042] [PMC 4157464]